Nephele is a web-based platform for microbiome data analysis. It was developed by scientists at the National
Institutes of Health to make microbiome analysis easier, streamlined, and more accessible for a variety of users.
Microbiome analysis is a growing area of science and medicine. It generates an enormous amount of raw data and requires advanced quantitative analysis. Technical capacity to analyze microbiome data – namely, analyzing, transferring, and storing biomedical "big data"— is a major challenge in this type of research. Nephele addresses these challenges through cloud-based computing, integrating open source microbiome data analysis and visualization tools into an intuitive user interface.
Like its namesake, a Greek mythological nymph who was molded from a cloud by Zeus, Nephele (pronounced ne-FEH-lee) is a powerful scientific tool created from a cloud-based environment.
For more information on Nephele, please see our publication on Nephele version 1.8. A publication of the current Nephele version 2 is coming soon. For changes from version 1.8 to version 2, please see the Release Notes. You can also find us on GitHub.
Nephele is targeted to researchers, sequencing facilities, students, and citizen scientists who would
like to perform standardized microbiome analyses on amplicon or shotgun sequencing data.
Nephele utilizes Amazon Web Services. If you are interested in system architecture, here is the overview of Nephele version 2.
For amplicon pipelines, i.e. 16S, ITS, Nephele generates Operational Taxonomic Unit (OTU) table with taxonomic associations for each OTU in standard biom format, plots for alpha and beta diversity, heatmaps, and tables of taxa counts of each taxonomic rank. For more detail, please see “how to make sense of output files”.